DIPLOMA IN MEDICAL LABORATORY TECHNOLOGY

BASIC COURSE ON MEDICAL LABORATORY TECHNOLOGY PART-I (First year) Paper-I Section-A (Anatomy) 1. Introduction to the subject - Anatomical position, common planes & Anatomical terms. -Different branches of Anatomy. 2. Histology -Typical animal cell (Structure & Function) -4 primary tissues (Classification & function) 3. Skeletal System - Axial and appendicular bones -Joints &movements 4. Skin, Fascia and Muscles & Tendons 5. Circulatory System –Heart & R.E.System -Spleen, Thymus & Tonsils 6. Respiratory System- Nose, Pharynx, Bronchi Lungs and Pleura 7. Digestive System- Alimentary canal (different parts)-Liver, Gall Bladder, Pancreases 8. Urogenital System- Different parts of urinary system -Different parts of Male & Female genital -System (Internal & External Genitalia) 9. Special Senses& General Sensibilities- Eye & Vision-Ears, Hearing & Equilibrium, -Taste. 10. Central & Peripheral nervous system- Brain & Spinal Cord. - Cranial & Spinal Nervous.- Autonomic Nervous System. 11. Regional Anatomy (Only Demonstration) – Extremities, Head & Neck, Thorax, Abdomen & Pelvis. Paper-I Section-B (Physiology) 1- Blood- Composition and general function of blood. Description of blood cells - normal counts & function. Steps of congratulation, Anticoagulants. Cerebrospinal Fluid, Formation, Composition & Function. Importance of blood groups composition & function of lymph. 2- Reparatory System -Name of structures involved in respirations and their function. External and internal respiration. How inspiration, expiration are brought about Transport of O2 and CO2 in the blood. Definition of respiratory rate, Tidal volume, vital capacity, Hypoxia. 3- Excretory System-Functions of Kidney, Nephron - Functions of Glomerulus and tubules, compositions of Urine, normal& abnormal. SkinFunction of Skin. 4- Digestive System-Composition and functions of saliva, mastication and deglutition. Functions of stomach, composition of gastric juice. Pancreatic Juice, Bile and Digestion of food by different Enzymes, Absorption and Defecation. 5- Endocrine-glands-Definition of endocrine gland, Names of the endocrine gland and the hormone secreted by them. Major actions of such Hormones. 6- Reproductive System-Name of primary and accessory organs in male and female. Name of secondary sexual characters in male and female. Function of ovary-formation of ova, actions of ovarian hormone, menstrual cycle. Functions of Testes-Spermatogenesis and secretions of testosterone. Fertilization Vasectomy and tubectomy. Paper-II Section-A (Community Medicine & Statistics) 1. Identification and Public Health Importance of arthropods (Entomology): Mosquitoes, Lice, Fleas, Flies, Rats & Rodents. 2. Water Sources: Types, Purification Bio-Medical Waste Management Sanitation in Public Health 3. Food and Nutrition: Collection of different food samples :Cereals, Pulses, Vegetables, Roots and tubers, Fats and oils, Animal foods including milk Food-borne diseases of Public Health importance, Assessment of Nutritional status. STATISTICS-GENERAL Mark-05 TABLES : Simple Tables, Frequency Distribution DIAGRAMS : Bar Diagrams, Histogram, Line Diagram Pie Diagram STATISTICAL AVERAGES : Mean, Median, Mode MEASURES OF DISPESION : Normal Curve, Range, Standard Deviation Standard Error. TESTS OF SIGNIFICANCE : 't' Test. Paper-II Section-B (COMPUTER) 1. Computer Basics: Importance, History, Computer Generation, Types of Computer, Anatomy of Computer, Input –output Devices, Processing Units and outline of Data Processing, Computer memory, external storage devices, Hardware, Software Basic functioning of Computers. 2. Computer and Communication, Networking, Internet 3. Use of computer in Radio-diagnosis/Pathology Laboratory Paper-II Section-C (Pharmacology) General Pharmacology 1. Drug, Drug nomenclature, Route of administration, concept of Pharmacokinetics, Pharmaco-dynamics and Adverse during action. 2. Drugs for the diseases of fundamental System GI System.Respiratory System.Cardiovascular System.Blood, Blood Coagulation, Thrombosis, different types of anti-coagula (Special emphasis).Drugs affecting the Urine and renal functions, excretion of drugs in stool, bile and other body fluids (Special emphasis). 3. Drugs for diseases of integrating systems of body Central Nervous System.Autonomic System.Endocrine System and autacoids. 4. Chemotherapeutic Agents Anti-Viral including AIDs, Hepatitis.Anti-Bacterial Drugs.Anti-Fungal Drugs. Anti-Protozoan Drugs.Anthelmintics.Anti-Cancer Drugs. 5. Antiseptic, disinfectants. 6. Drugs interfering in different Pathological tests. 7. Measurement of Drug levels in different body fluids and significance. Paper-III BIOCHEMISTRY 1. Chemistry of a) Carbohydrates including proctiglycon b) Fat c) Protein & Amino acid 2. Water & Fat soluble Vitamin. 3. Enzymes (Classification, factors regulating, inhibitors 2 clinical application) 4. Buffers, Molarily, indicators, Radioisotopes, Radiation hazard, RA. 5. Overview of Iron, Calcium, Iodine, Flourine. 6. Overview of Nucleic Acids & Uric Acid. BASIC COURSE ON MEDICAL LABORATORY TECHNOLOGY PART-II (Final year) PAPER–I PATHOLOGY General Pathology Cell injury, inflammation & repair adaptation, hemodynamics, infectious diseases, nutritional diseases, genetic diseases, neoplasia and occupational diseases. Hematology 1. Hemoglobin estimation, anemia classification. 2. Blood group ABO/Rh typing, cross matching, complications of mismatch transfusion, selection of donor, mandatory tests, comb’s test, component separation, preservation and uses. 3. Transfusion transmitted diseases. HISTOTECHNOLOGY, CYTOLOGY, MUSEUM STUDY Methods of examination of tissues and cells, Fixation of tissue: Classification of fixatives, Simple Fixatives and their properties, Tissue processing, Collection of specimen, Labeling and fixation, Dehydration, Clearing, Impregnation, Embedding, Paraffin block making, Section Cutting, Microtomes and microtome knives – sharpening of knife, Microtome use – Honing, Stropping, Techniques of section cutting, Mounting of sections, Frozen section. (a) Staining: Dyes and their properties, Theory of staining, staining technique with haematoxylin and eosin, Mounting of actions, Common special stains, Routine H & E, Meason Trichrome, Men – Geison, Reticulin, PAS, Fe, Lipid, Mucicamine, Vencos for calcium, Special staining, Decalcification, Fixation, Decalcification, Detection of end point, Neutralization and processing. (a) Exfoliative Cytology and Fine needle aspiration cytology Types of specimens and preservation, Preparation and fixation of smears, Papanicolaous staining technique/MCC staining/HE staining/, Sex chromatin staining, Nuscum Techniques, Reception of specimen., Preparation of fixation , Preservation , Presentation AUTOPSY TECHNIQUE: Assisting in autopsy, Preservation of organs and Processing of the tissue. 1. Waste disposal and safety in laboratory. Histotechnology and Cytology, Fixation, processing, embedding and section and, reparation of slides., Sharpending of the knife. , Preparation of fixatives and, decalcifying fluid. , Preparation of adhesives to fix the section to the slide. , Preparation and fixation of cytology smears and Papanicolaoue’s staining techniques., MOG staining /HE staining. , Mounting. PAPER–II MICROBIOLOGY Gen. Bacteriology Syst. Bacteriology Clin. Microbiology Mycology IMMUNOLOGY AND SEROLOGY Emphasis on principal and uses/application ,Immunity –Basic principles and classification, Antigen, Antibody (Immunoglobulin’s), Complement system, Antigen – Antibody reactions, Hypersensitivity- classification & different skin tests used for diagnosis., Immunodeficiency diseases including AIDS – in brief, Autoimmunity – Basic concept, Immuno-prophylaxis & Immunization schedule, Vaccines-classification & uses. PARASITOLOGY - Introduction & classification of medically important parasites, Intestinal & Tissue protozoa (E.histolylica, Giardia Primary Amoebic meningoencephalitis) - Malaria parasite, Leishmanial parasites, Tapeworms, Flukes of liver and , Intestine, Intestinal nematodes, Filarial worms and other tissue nematodes VIROLOGY - General Characters of viruses, Classification in brief and name of the diseases they produce., Hepatitis viruses, HIV, (Polio, Rabies, Rata, Measles, Dengue) - Oncogenic viruses in brief, Collection and transport of virological specimens - Laboratory diagnosis of viral infections (various methods of virus culture, serology etc.) PAPER–III BIOCHEMISTRY 1. Glucose Homeostasis, overview DM, HGAIC. 2. Lipoprotein & Hyper Lipoprotein. 3. Liver function test. 4. Renal function test. 5. Thyroid function test. 6. Alimentary function test. 7. Water & Electrolytic Balance. ORGAN FUNCTION TESTS 1. Thyroid Function Tests 2. Renal FunctionTests 24 hr collection, preservation Physical characteristics, clearance tests. 3. Liver function tests 4. Gastric Function Tests 5. Pancreatic Function Tests Serum Amylase, Serum Trypsin, Serum Lipase, 6. Biochemical tests of CSF. 7. Instrumentation ORAL AND PRACTICAL List of Practical’s in Clinical Bio-chemistry 1. Principals of colorimetry & overview of semi analyzer. 2. Determination of Glucose in Blood/Serum, Glucose Tolerance Tests 3. Quantition of Urea 4. Quantition of Creatinine 5. Quantition of Uric Acid 6. Cholesterol, Triglycerides, HDL Cholesterol, Lipid Profile (Demonstration Only) 7. Total serum protein and albumin 8. T3, T4, TSH (Data Interpretation Only) 9. Demonstrations of Electrophoresis & chromatogram